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KMID : 0369820050350020089
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Jorunal of Korean Pharmaceutical Sciences
2005 Volume.35 No. 2 p.89 ~ p.94
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Preparation and Characterization of Lysozyme Nanoparticles using Solution Enhanced Dispersion by Supercritical Fluid (SEDS) Process
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김동현/Kim DH
박희준/강선호/전승욱/김민수/이시범/박정숙/황성주/Park HJ/Kang SH/Jun SW/Kim MS/Lee SB/Park JS/Hwang SJ
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Abstract
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The micron or nano-sized lysozyme as a model protein drug was prepared using solution enhanced dispersion by supercritical fluid (SEDS) process at various conditions (e.g., solvent, temperature and pressure) to investigate the feasibility of pulmonary protein drug delivery. The lysozyme particles prepared were characterized by laser diffraction particle size analyzer, scanning electron microscopy (SEM) and powder X-ray diffractometry (PXRD). The biological activity of lysozyme particles after/before SEDS process was also examined. Lysozyme was precipitated as spherical particles. The precipitated particles consisted of 100 -200 nm particles. Particle size showed the precipitates to be agglomerates with primary particles of size 1 - 5 μm. The biological activity varied between 38 and 98% depending on the experimental conditions. There was no significant difference between untreated lysozyme and lysozyme after SEDS process in PXRD analysis. Therefore, the SEDS process could be a novel method to prepare micron or nano-sized lysozyme particles, with minimal loss of biological activity, for the pulmonary delivery of protein drug.
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KEYWORD
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Supercritical fluid, Solution enhanced dispersion by supercritical fluid (SEDS), Lysozyme, Nanoparticle, Pulmonary delivery
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